Chromotek/Nuclear Actin-Chromobody

Chromotek/Nuclear Actin-Chromobody

螞蟻淘常見平行進(jìn)口產(chǎn)品有：

Thermofisher旗下Gibco培養(yǎng)系列、ABI細(xì)胞計(jì)數(shù)儀及PCR儀、Invitrogen分子生物學(xué)試劑及Qubit3.0、Ambion的RNA相關(guān)試劑、Molecular probes試劑、Pierce蛋白相關(guān)試劑盒、Nanodrop One/OneC；Parafilm封口膜；Viskase透析袋；Sigma-Aldrich試劑；AccuStandard 標(biāo)準(zhǔn)品；Millipore抗體；Glen Research 核酸合成試劑；Bio-Rad電泳儀及PCR儀；Nanocs的PEG聚合物；  Chromotek羊駝抗體；   Polysciences轉(zhuǎn)染試劑；  Echelon-inc 脂類研究試劑盒；   R&D Systems抗體及Elisa試劑盒； Jackson二抗及封閉血清； Biotium 熒光染料；NEB內(nèi)切酶及NGS相關(guān)產(chǎn)品；  Athens Research & Technology人類蛋白及抗血清；  Illumina二代測試試劑盒； Tocris 小分子試劑； TwistDx恒溫?cái)U(kuò)增試劑盒（RPA）； Enzymatics二代測序酶；Novus抗體及Elisa試劑盒；AG scientific潮霉素B、G-418；Plantmedia植物培養(yǎng)系列及抗體；Agilent 2100生物分析儀；Biolog耗材；Biomatik的Elisa試劑盒；Covaris基因打斷儀；CST抗體；Dharmacon的siRNA產(chǎn)品。

Chromotek/Nuclear Actin-Chromobody

　　Visualize the actin dynamics in real time in the nucleus with the nuclear Actin-Chromobody®. Upon transformation, your cells express the nuclear Actin-Chromobody® and transport the nuclear Actin-Chromobody into the nucleus. The nuclear cytoskeleton is visualized in real time by binding of the fluorescent Chromobody® to Actin.Actin-Chromobody®

　　Courtesy of Matthias PlessnerNIH 3T3 cells (murine fibroblasts) transfected with the nuclear Actin-Chromobody® (TagGFP2, green) and the Lamin-Chromobody® (TagRFP2, red) were starved in serum-free medium for 24 hours. Nuclear Actin-Chromobody-probed endogenous F-actin assembly and distribution is shown 30 s after stimulation with serum.

　　Trace actin dynamics in cell nuclei non-invasively

　　With the nuclear Actin-Chromobody® plasmid you obtain the sequence of the Alpaca antibody to Actin, also termed VHH-Actin or Actin-nanobody, fused to TagGFP (Evrogen) and a nuclear localization sequence (NLS). The transient binding does not influence cell viability or motility and thus offers you the unique possibility to observe the cytoskeleton in your cell nuclei.

　　The nuclear Actin-Chromobody® plasmid has been published and described by Prof. Dr. Robert Grosse, Institute of Pharmacology, BPC, University of Marburg, Germany under license from ChromoTek:

　　Matthias Plessner, Michael Melak, Pilar Chinchilla, Christian Baarlink, Robert Grosse (2015). Nuclear F-actin formation and reorganization upon cell spreading J Biol Chem. (PubMed)